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Key to the Common Genera of Freshwater Diatoms

1a. Radially symmetrical in valve view (valves can appear rectangular if in girdle view) [CENTRALES] 2
1b. Not radially symmetrical in valve view [PENNALES] 11

Centrales

2a. Valves with many intercalary bands (barely distinguishable), valves in girdle view appearing rectangular and flattened, weakly silicified, normally planktonic 10
2b. Valves appearing otherwise 3
3a. Valves with two large ocelli (appearing like bubbles at the margin) Pleurosira
3b. Valves appearing otherwise 4
4a. Valves usually appearing in side view (therefore appear rectangular), cells usually forming chains of varying number 5
4b. Valves disc to drum shaped, valve mantles and girdle mostly reduced, cells generally single 6
5a. Valve mantle and valve without detail, areolae on the mantle just distinguishable with light microscope Melosira
5b. Valve mantle with easily distinguishable areolae Aulacoseira (part)
6a. Valves with a sulcus (a distinct ring when focused through) Aulacoseira (part)
6b. Valves without a sulcus 7
7a. Valves without distinctive features Melosira
7b. Valves otherwise 8
8a. Areolae arranged radially around the valve margin , usually not extending to the center of the valve, central area distinct Cyclotella
8b. Central area otherwise 9
9a. Areolae arranged in clear radial lines extending to valve centre Cyclostephanoid group
  (Stephanodiscus, Cyclostephanos, Actinocyclus, Thallassiosira)
9b. Areolae arranged otherwise Actinocylcus , Thalassiosira
10a. Valves with one long bristle/seta at both ends Urosolenia
10b. Valves with two long bristles/seta at both ends Acanthoceras

Pennales

11a. Valves without raphes 12
11b. Valves with raphes 20

Araphidae

12a. Valves without striae Diatoma
12b. Valves with striae 13

13a. Valves heteropolar (may only be slightly so) 14
13b. Valves isopolar 15

14a. Valves swollen at both ends Asterionella
14b. Valves swollen at head end only Meridion

15a. Valve swollen centrally and at ends, with septa inside frustule Tabellaria
15b. Valve otherwise 16

16a. Clearly punctate striae, distinct hyaline central area Ctenophora
16b. Valve otherwise 17

17a. Shortened striae (<half of valve width) 18
17b. Valve otherwise 19

18a. Valves longer than 20 um Tabularia
18b. Valves shorter Staurosira group (part)

19a. Valves > 20 um; if shorter then with clearly defined central area Fragilaria /Synedra
19b. Valves shorter Staurosira group (part)
(it is difficult to separate Fragilaria, Synedra and Staurosira 'group' in cleared material)

20a. Valves with only vestigal raphes at distal ends on ventral side [PSEUDORAPHIDAE] Eunotia
20b. Valves with at least one true raphe 21

21a. Valves with raphes on one valve only [MONORAPHIDAE] 22
21b. Valves with raphes on both valves [BIRAPHIDAE] 24

Monoraphidae

22a. Valves bent in girdle view 23
22b. Valves +/- flat so rarely seen in girdle view Cocconeis

23a. Coarsely punctate; brackish water forms Achnanthes
23b. At most finely punctate; generally freshwater Achnanthidium

Biraphidae

24a. Valves bent in girdle view; heteropolar, vestigal raphe on one valve Rhoicosphenia abbreviata
24b. Valves straight 25

25a. Valves with heavy costae 26
25b. Valves without costae 31

26a. Valves completely crossed by costae 27
26b. Valves not completely crossed by costae 29

27a. Valves dorsoventral 28
27b. Valves otherwise Denticula

28a. Raphe lying along ventral side rising dorsally at centre Epithemia
28b. Raphe lying along dorsal side Rhopalodia

29a. Valves saddle-shaped Campylodiscus
29b. Valves otherwise 30

30a. Valves transapically undulate Cymatopleura
30b. Valves otherwise Surirella

31a. Raphe in a canal crossed by fibulae 32
31b. Raphe appearing otherwise 36

32a. Raphe canal spiralling down length of valve Cylindrotheca
32b. Raphe straight 33

33a. Raphe canal central Bacillaria
33b. Raphe usually lateral 34

34a. Valve with longitudinal hyaline fold Tryblionella
34b. Valve appearing otherwise 35

35a. Raphe canals on same side of each valve Hantzschia
35b. Raphe canals on opposite side of each valve Nitzschia

36a. Valve heteropolar Gomphonema
36b. Valve isopolar 37

37a. Valve dorsoventral 38
38b. Valve appearing otherwise 41

38a. Ventral side with swollen central area Reimeria
38b. Ventral side otherwise 39

39a. Valve mantle usually much wider at dorsal side, often with many intercalary bands Amphora
39b. Dorsal side only slightly wider 40

40a. Raphe rising from ends to valve centre; distal raphe ends deflected dorsally Cymbella
40b. Raphe +/- parallel with ventral side not rising to centre; distal raphe ends deflected ventrally Encyonema

41a. Valve in S shape 42
41b. Valve appearing otherwise 43

42a. Transverse and longitudinal striae cross at right angles Gyrosigma
42b. Valve with diagonal striae Pleurosigma

43a. Valve apically twisted with raphe along margins of 'wings' Entomoneis
43b. Valve appearing otherwise 44

44a. Locules (chambers) on valve margin usually evident (also wavy raphe and punctate striae) Mastogloia
44b. Locules never present 45

45a. Most valves <20 um; isolated stigma in central area Luticola
45b. Stigma never present 46

46a. Raphe in heavy rib, ending in an 'arrow' 47
46b. Axial area may be wide but not with rib 48

47a. Proximal raphe ends widely separated Berkeleya
47b. Valve otherwise Frustulia

48a. Striae interrupted forming longitudinal lines  
48b. Striae complete without longitudinal lines 53

49a. Longitudinal lines hyaline, centrally constricted Fallacia
49b. Characteristic untrue 50

50a. Raphe lying in thickened axial area Diploneis
50b. Characteristic untrue 51

51a. Striae punctate and incomplete forming several wavy longitudinal lines Anomoneis
51b. Striae appearing otherwise 52

52a. Striae generally clearly punctate and proximal raphe ends usually deflected in opposite directions Nedium
52b. Striae appearing otherwise Caloneis

53a. Valve with clear central area extending to margin 54
53b. Valve appearing otherwise 56

54a. Striae punctate, sometimes not obviously so 55
54b. Striae otherwise Pinnularia& Caloneis (part)

55a. Cells rounded often appearing on their side Staurophora
55b. Valve face relatively flat Stauroneis

56a. One valve with craticulum, usually with parallel striae extending to axial area Craticula [KEY]
56b. Valve appearing otherwise 57

57a. Valve apices hyaline, extending to valve margin Sellophora
57b. Valve appearing otherwise 58

58a. Striae alveolate Pinnularia & Caloneis (part)
58b. Valve appearing otherwise Navicula [KEY]

For further information or comments on the generic key, please contact Dr Peter Gell
Created by Jason Sonneman, last modified 13/07/99

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